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在单因素试验基础上,研究了响应曲面法优化氧化亚铁硫杆菌氧化Fe2+的工艺条件。结果表明,初始ρ(Fe2+)、初始pH值、培养温度、接种量与响应值Fe2+氧化速率有显著相关性。典型性分析得到氧化亚铁硫杆菌氧化Fe2+的最佳工艺条件为:初始ρ(Fe2+)为8.44 g/L,溶液初始pH值为2.1,培养温度为33℃,接种量为12%。在此条件下,Fe2+氧化速率理论值达到0.217 g/(L.h),验证试验条件下实际最大氧化速率为0.215 g/(L.h)。
Abstract：Based on single factor experiments,optimizing process conditions of oxidizing Fe2+ using thiobacillus ferrooxidants by Response Surface Design Method(RSM) were researched.The results showed that there were significant correlation between initial concentration of Fe2+,initial value of pH,culture temperature,inoculum size and response value——oxidation rate of Fe2+.The optimum conditions of oxidizing Fe2+ by thiobacillus ferrooxidants were obtained through typical analysis that the initial concentration of Fe2+ is of 8.44 g/L,the initial value of pH is of 2.1,the culture temperature is of 33 ℃ and the inoculum size is of 12%.On these conditions,theoretical value of the oxidation rate reached 0.217 g/(L·h).The actual maximum oxidation rate which was verified at the test conditions,was of 0.215 g/(L·h).
[1]邓恩建,杨朝晖,曾光明,等.氧化亚铁硫杆菌的研究概况[J].黄金科学技术,2005,13(9):9-11.
[2]杨显万,沈庆峰,郭玉霞.微生物湿法冶金[M].北京:冶金工业出版社,2003.
[3]王昌汉.矿业微生物与铀铜金等细菌浸出[M].长沙:中南大学出版社,2003.
[4]高仁喜.国内外铀、金矿石微生物浸出的应用研究[J].铀冶金,1996(4):7-16.
[5]吴有炜.试验设计与数据处理[M].苏州:苏州大学出版社,2002.
[6]崔凤杰,许泓瑜,舒畅,等.响应曲面法优化灰树花水溶性多糖提取工艺的研究[J].食品科学,2006,27(4):142-146.
[7]李鹰,刘峙嵘,戴荧.微污染水混凝最优水力条件研究[J].湿法冶金,2010,29(2):108-113.
[8]Muralidhar R V,Chirumamila R,Marehant R,et al.AResponse Surface Approach for the Comparison of LipaseProduction by Candida Cylindracea Using Two DifferentCarbon Sources[J].Biochemical Engineering Journal,2001,9(1):17-23.
基本信息:
DOI：10.13355/j.cnki.sfyj.2011.01.007
中图分类号:TF18
引用信息:
[1]李红,胡兆吉,董庆珍,等.响应曲面法优化氧化亚铁硫杆菌氧化Fe~(2+)工艺条件试验研究[J].湿法冶金,2011,30(01):14-19.DOI:10.13355/j.cnki.sfyj.2011.01.007.
2011-03-15
2011-03-15